光遗传学
神经科学
生物
神经发生
神经元
同源盒
索马
转录因子
运动神经元
秀丽隐杆线虫
生物神经网络
细胞生物学
基因
遗传学
脊髓
作者
Manho Lim,Jyothsna Chitturi,Valeriya Laskova,Jun Meng,Daniel Findeis,Anne Wiekenberg,Ben Mulcahy,Linjiao Luo,Yan Li,Yangning Lu,Wesley Hung,Yixin Qu,Chi-Yip Ho,Douglas Holmyard,Ni Ji,Rebecca McWhirter,Aravinthan D. T. Samuel,David M. Miller,Ralf Schnabel,John A. Calarco,Mei Zhen
出处
期刊:eLife
[eLife Sciences Publications, Ltd.]
日期:2016-11-18
卷期号:5
被引量:40
摘要
Neuromodulators shape neural circuit dynamics. Combining electron microscopy, genetics, transcriptome profiling, calcium imaging, and optogenetics, we discovered a peptidergic neuron that modulates C. elegans motor circuit dynamics. The Six/SO-family homeobox transcription factor UNC-39 governs lineage-specific neurogenesis to give rise to a neuron RID. RID bears the anatomic hallmarks of a specialized endocrine neuron: it harbors near-exclusive dense core vesicles that cluster periodically along the axon, and expresses multiple neuropeptides, including the FMRF-amide-related FLP-14. RID activity increases during forward movement. Ablating RID reduces the sustainability of forward movement, a phenotype partially recapitulated by removing FLP-14. Optogenetic depolarization of RID prolongs forward movement, an effect reduced in the absence of FLP-14. Together, these results establish the role of a neuroendocrine cell RID in sustaining a specific behavioral state in C. elegans.
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