This study introduces an easy method of pre- paring xyloglucan hydrogel from xyloglucan, which is purified from tamarind seed gum. Xyloglucan hydrogel was prepared in 2 wt% solution by treating with b-galactosi- dase. Physical and chemical properties (molecular mass, size and viscosity) of xyloglucan hydrogel and xyloglucan solution were tested for a comparison. Experiments of drug release in vitro and in vivo were operated to investigate the potentialities of xyloglucan hydrogel as the biomedical sustained-release carriers for drug delivery system.