Evaluation of Avidin-Biotin Binding Using a Biotin Labeled with Daunomycin by Photoacoustic Spectrometry

Abstract A method of evaluation for avidin-biotin binding using photoacoustic spectrometry (PAS) was developed. Biotin labeled with daunomycin was prepared. The avidin-biotin binding between avidin and the labeled biotin (LB) makes avidin colored. This is because the part of daunomycin is taken in biotin sites of avidin. Therefore, the concentration of avidin could be estimated based on measurement of the LB with avidin by PAS.