脂肪酸
多不饱和脂肪酸
食品科学
亚油酸
大豆油
生物化学
饱和脂肪酸
甘油
作者
Alfonso Robles,María J. Jiménez,Luis Esteban,Pedro A. González,L. Martín,A. Rodríguez,E. Molina
出处
期刊:Lwt - Food Science and Technology
日期:2011-12-01
卷期号:44 (10): 1986-1992
被引量:29
标识
DOI:10.1016/j.lwt.2011.05.022
摘要
Abstract This paper studies the synthesis of structured triacylglycerols (STAGs) rich in palmitic and docosahexaenoic acids (PA and DHA) at sn -2 position and oleic acid (OA) at sn -1,3 positions by a four step process. First, triacylglycerols (TAGs) were obtained with 63–66 mol PA/100 mol total fatty acids and 10 mol DHA/100 mol by acidolysis of tuna oil and commercial PA, catalyzed by the non-positionally specific lipase Novozym 435. Then these TAGs were purified neutralizing the free fatty acids (FFAs) by KOH hydroethanolic solutions and extracting TAGs with hexane; these TAGs were completely recovered as pure TAGs (without FFAs). The third step involved the displacement of fatty acids located at sn- 1,3 positions by acidolysis of PA and DHA enriched TAGs with OA rich FFAs, catalyzed by the sn- 1,3 specific lipase DF from Rhizopus oryzae, immobilized on Accurel MP-1000; TAGs with 67 mol OA/100 mol at sn -1,3 positions and 52.1 and 15.4 mol PA and DHA, respectively, per 100 mol at sn -2 position were obtained. Both acidolysis reactions were carried out in stirred tank reactors (STRs) with lipase both dispersed in the reaction medium and contained in a cartridge filter attached to the stirrer rod. Finally STAGs were purified and obtained with yields of over 80 mol STAGs/100 mol STAGs in the reaction product (no FFAs were detected).
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