增殖性玻璃体视网膜病变
蛋白质组
玻璃体切除术
蛋白质组学
扁平部
视网膜脱离
化学
医学
生物
细胞生物学
病理
眼科
视网膜
生物化学
视力
基因
作者
Jing Yu,Feng Liu,Shu-Jian Cui,Yan Liu,Zhenlong Song,Hui Cao,Fenge Chen,Weijun Wang,Tao Sun,Fang Wang
出处
期刊:Proteomics
[Wiley]
日期:2008-09-01
卷期号:8 (17): 3667-3678
被引量:56
标识
DOI:10.1002/pmic.200700824
摘要
Proliferative vitreoretinopathy (PVR) is the most common cause of anatomic failure in retinal detachment surgery. To understand the molecular mechanisms, vitreous proteomes of patients with PVR were investigated by two-dimensional-nano-liquid chromatography coupled with tandem mass spectrometry. Vitreous samples of moderate PVR (grade B), and severe PVR (grade C or D) were aspirated during pars plana vitrectomy before infusion. In the current study, 129, 97 and 137 proteins were identified in vitreous of normal control, moderate and severe PVR, respectively. In PVR vitreous samples, complement components, serine proteinase inhibitors, and extracellular proteins were up-regulated or appeared, while normal cytoskeleton and metabolism proteins were down-regulated or disappeared. It was noteworthy that the proteins involved in transcription and translation regulation increased in vitreous with PVR. Among 102 PVR-specific proteins, kininogen 1 was specifically detected in both vitreous and the corresponding serum. Therefore, it can be concluded that PVR is a complicated pathology process with great amount of proteins involved in metabolism dysfunction, immune reactions, and cytoskeleton remolding. Kininogen 1 may be a candidate biomarker of PVR. Further investigations of these special proteins will provide additional targets for treatment or prevention of ocular proliferative diseases.
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