幽灵蛋白
耳蜗
螺旋神经节
传出的
毛细胞
立体纤毛(内耳)
生物
神经科学
基因剔除小鼠
内耳
细胞生物学
解剖
化学
细胞骨架
细胞
传入的
基因
遗传学
作者
Michael C. Stankewich,Jun-Ping Bai,Paul R. Stabach,Saaim Khan,Winston Tan,Alexei Surguchev,Lei Song,Jon S. Morrow,Joseph Santos-Sacchi,Dhasakumar Navaratnam
标识
DOI:10.1016/j.heares.2022.108564
摘要
Reports have proposed a putative role for βV spectrin in outer hair cells (OHCs) of the cochlea. In an ongoing investigation of the role of the cytoskeleton in electromotility, we tested mice with a targeted exon deletion of βV spectrin (Spnb5), and unexpectedly find that Spnb5(-/-) animals' auditory thresholds are unaffected. Similarly, these mice have normal OHC electromechanical activity (otoacoustic emissions) and non-linear capacitance. In contrast, magnitudes of auditory brainstem response (ABR) wave 1-amplitudes are significantly reduced. Evidence of a synaptopathy was absent with normal hair cell CtBP2 counts. In Spnb5(-/-) mice, the number of afferent and efferent nerve fibers is decreased. Consistent with this data, Spnb5 mRNA is present in Type I and II spiral ganglion neurons, but undetectable in OHCs. Together, these data establish that βV spectrin is important for hearing, affecting neuronal structure and function. Significantly, these data support that βV spectrin as is not functionally important to OHCs as has been previously suggested.
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