胶质瘤
基因敲除
癌症研究
泛素
异位表达
脱氮酶
生物
细胞生物学
细胞凋亡
细胞培养
生物化学
基因
遗传学
作者
Xingchen Zhao,Ming Zhou,Yong Yang,Minjie Luo
出处
期刊:Bioengineered
[Informa]
日期:2021-12-19
卷期号:12 (2): 12636-12645
被引量:22
标识
DOI:10.1080/21655979.2021.2011633
摘要
The ubiquitin hydrolase OTUB1 has been elucidated to be highly expressed in tumors, however, its roles in glioma progression are still confusing. Here, via analyzing several online datasets, OTUB1 expression was shown to be remarkably increased in glioma tissues compared to that in the adjacent tissues, and predicted a poor overall survival of glioma patients. Then OTUB1 was knocked down in glioma cells and it was found that OTUB1 knockdown significantly reduced glioma cell stemness by detecting sphere-formation ability, stemness marker expression, and ALDH activity. Mechanistic experiments revealed that OTUB1 stabilized SLC7A11 protein via directly interacting with SLC7A11, which is a key suppressor of ferripotosis. Indeed, OTUB1 knockdown triggered ferroptosis dependent on SLC7A11 expression. Notably, ectopic expression of SLC7A11 attenuated the inhibition of OTUB1 knockdown on the stemenss of glioma cells. Finally, we found a positive correlation between OTUB1 and SLC7A11 expression in clinical samples. Taken together, this work identifies a novel OTUB1/SLC7A11 axis contributing to glioma cell stemness.
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