SF/PVP nanofiber wound dressings loaded with phlorizin: preparation, characterization, in vivo and in vitro evaluation

Electrospinning-based wound dressings have multiple functions such as antibacterial, anti-inflammatory, and therapeutic, and are important in skin wound care. Herein, we designed a phlorizin (PHL)-loaded silk protein/polyvinylpyrrolidone (SF/PVP) composite nanofibrous membrane, which can be used as multiple wound dressings. In particular, SF/PVP/PHL scaffolds have high porosity and mechanical properties, exhibiting suitable permeability and hydrophilicity. The SF/PVP/PHL scaffolds containing PHL also have excellent antibacterial and antioxidant activities. Furthermore, the nanofiber significantly accelerated the wound healing process in a full-thickness skin injury model by enhancing wound re-epithelialization and collagen deposition density, increasing the content of macrophage antigen (CD68), platelet-endothelial cell adhesion molecule (CD31), proliferating cell nuclear antigen (PCNA) and inhibiting the expression of α-smooth muscle actin (α-SMA) at the wound site. The mechanism may be related to the inhibition of activation of phosphatidylinositol 3-kinase/serine-threonine kinase/ target of rapamycin (PI3K/AKT/mTOR) signaling pathway to enhance autophagy. Therefore, SF/PVP/PHL nanofibers can ideally meet the various requirements of the wound healing process and are promising wound dressing candidates for future clinical applications.