化学
代谢组学
中医药
新陈代谢
行为绝望测验
气相色谱-质谱法
药理学
偏最小二乘回归
代谢途径
生物化学
色谱法
质谱法
内科学
抗抑郁药
统计
医学
病理
替代医学
数学
海马体
作者
Xiaoyao Miao,Bingkun Xiao,Sufang Shui,Jianyun Yang,Renfang Huang,Jun-Xing Dong
标识
DOI:10.1016/j.jpba.2018.01.028
摘要
Danggui Buxue Tang (DBT), believed to invigorate 'Qi' (vital energy) and nourish 'Blood' (body circulation), is a traditional Chinese medicine formula. In this study, a metabolomics approach with gas chromatography coupled to mass spectrometry combined with pattern recognition was adopted to investigate the underlying mechanism of the antifatigue effect of DBT on fatigue of mice induced by weight-loaded forced swimming. Fourteen endogenous metabolites, up-regulated or down-regulated, were identified in the model mice by analysis tools of partial least-squares discriminant analysis (PLS-DA) and XCMS online software. Furthermore, the metabolites were reversed by DBT treatment, offering evidence for the antifatigue effect. In addition, intervention of DBT changed the levels of biochemical parameters. DBT showed obvious efficacy on the fatigued mice possibly by regulating the pathways of phenylalanine, tyrosine and tryptophan metabolism, glycine, serine, and threonine metabolism, glyoxylate and dicarboxylate metabolism, pyruvate metabolism, and TCA cycle. This study demonstrated that DBT has a good antifatigue effect and that metabolomics is a powerful means to gain insights into the therapeutic effect of traditional Chinese medicine formulas.
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