鞭毛蛋白
银纳米粒子
细菌
鞭毛
纳米颗粒
铜绿假单胞菌
大肠杆菌
抗生素耐药性
微生物学
纳米技术
抗菌剂
材料科学
化学
抗生素
生物
生物化学
基因
遗传学
作者
Aleš Panáček,Libor Kvı́tek,Monika Smékalová,Renata Večeřová,Milan Kolář,Magdaléna Röderová,Filip Dyčka,Marek Šebela,Robert Prucek,Ondřej Tomanec,Radek Zbořil
标识
DOI:10.1038/s41565-017-0013-y
摘要
Silver nanoparticles have already been successfully applied in various biomedical and antimicrobial technologies and products used in everyday life. Although bacterial resistance to antibiotics has been extensively discussed in the literature, the possible development of resistance to silver nanoparticles has not been fully explored. We report that the Gram-negative bacteria Escherichia coli 013, Pseudomonas aeruginosa CCM 3955 and E. coli CCM 3954 can develop resistance to silver nanoparticles after repeated exposure. The resistance stems from the production of the adhesive flagellum protein flagellin, which triggers the aggregation of the nanoparticles. This resistance evolves without any genetic changes; only phenotypic change is needed to reduce the nanoparticles’ colloidal stability and thus eliminate their antibacterial activity. The resistance mechanism cannot be overcome by additional stabilization of silver nanoparticles using surfactants or polymers. It is, however, strongly suppressed by inhibiting flagellin production with pomegranate rind extract.
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