适体
化学
卡那霉素
检出限
胶体金
色谱法
壳聚糖
低聚糖
组合化学
线性范围
选择性
小分子
纳米颗粒
纳米技术
生物化学
分子生物学
抗生素
材料科学
催化作用
生物
作者
Xiaoyan Qi,Yinglin Zhao,Haipeng Su,Xun Hu,Ling Li,Rui Ma,Xiaochen Yan,Jianan Sun,Qianqian Wang,Xiangzhao Mao
出处
期刊:Talanta
[Elsevier]
日期:2022-02-01
卷期号:238: 123032-123032
被引量:31
标识
DOI:10.1016/j.talanta.2021.123032
摘要
Herein, the split aptamers, chitosan oligosaccharide, and AuNPs were combined as nanocomposites that present different formations to develop a label-free colorimetric aptasensor for rapid detection of small molecules. Kanamycin was chosen as a model target. Computational studies were performed to assist in the design of orientated immobilization of the split aptamers onto the AuNPs surface. Chitosan oligosaccharide was initially applied as an aggregation inducer of AuNPs, and chitopentaose was screened as the optimal. Under optimized conditions, the proposed aptasensor showed high sensitivity and selectivity, with a limit of detection of 20.58 nM, a linear range of 25-800 nM, and good recoveries of 98.49-104.9% and 85.69-107.0% when employed to detect kanamycin in tap water and milk samples, respectively. Only 55 min was needed for the whole assay. More importantly, this study can serve as a novel and robust reference for the aptasensing detection of other small molecules.
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