干细胞
细胞生物学
造血
生物
骨髓
成体干细胞
细胞分化
成骨细胞
移植
造血干细胞
免疫学
体外
内皮干细胞
医学
内科学
遗传学
基因
作者
Teruyuki Kajiume,Yumi Kawahara,Louis Yuge,Masao Kobayashi
出处
期刊:Stem cell investigation
[AME Publishing Company]
日期:2021-10-01
卷期号:8: 21-21
被引量:5
标识
DOI:10.21037/sci-2021-019
摘要
Intrinsic factors related to self-renewal regulatory factors in hematopoietic stem cells are well known; however, limited information is available on extrinsic factors, such as the cell environment. Therefore, in this study, we analyzed the regulatory mechanism of hematopoietic stem cell self-renewal, focusing on the osteoblastic niche, and examined how adherence to osteoblasts affects stem cell differentiation.For this experimental study, we developed a co-culture system for hematopoietic stem cells and osteoblasts, such that cells adhered to osteoblasts can be separated from those that do not. Murine Sca1-positive cells were separated into groups according to whether they were attached to osteoblasts or detached from osteoblasts, and each group was then subjected to colony assays and bone marrow transplantation experiments.Adhered Sca1-positive cells developed more secondary colonies than non-adhered Sca1-positive cells. Furthermore, in bone marrow transplantation experiments, adhered Sca1-positive cells showed successful engraftment. We explored the role of Polycomb genes in the regulation of cell fate and found that self-renewing cells attached to osteoblasts had high Bmi-1 expression and low Mel-18 expression, while this expression was reversed in differentiating cells.Our results suggest that hematopoietic stem cells self-renew when they remain in osteoblastic niches after cell division. Further, when stem cells leave the niches, they undergo differentiation.
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