电化学发光
鲁米诺
化学
检出限
发光体
氧化还原
线性范围
光化学
化学发光
信号(编程语言)
锰
材料科学
光电子学
发光
无机化学
色谱法
有机化学
程序设计语言
计算机科学
作者
Jinna Guo,Mingsen Xie,Peiyao Du,Yu Liu,Xiaoquan Lu
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2021-07-20
卷期号:93 (30): 10619-10626
被引量:27
标识
DOI:10.1021/acs.analchem.1c01891
摘要
Luminol, as a classical luminophore, plays a crucial role in electrochemiluminescence (ECL). However, the traditional luminol–H2O2 ECL system suffers from the self-decomposition of H2O2 at ambient temperature, which hinders its further application in quantitative analysis. In this work, for the first time, we developed atomically gold-supported two-dimensional VO2 nanobelts (Au/VO2) as an advanced co-reaction promoter to speed up the reduction of dissolved oxygen to superoxide radicals (O2•–), which react with the luminol anion radical and greatly promote the ECL emission. The ECL resonance energy transfer (ECL-RET) between the hollow manganese dioxide nanospheres and luminol results in a conspicuously decreased ECL signal response, and in the presence of glutathione (GSH), effective redox reaction between manganese dioxide and GSH restores the ECL signal. As a consequence, the designed sensor based on ECL-RET-assisted Au/VO2 signal amplification showed outstanding performance for “signal-on” detection of GSH in the concentration range of 10–3 to 10–10 M, and the detection limit was as low as 0.03 nM. The ECL sensor displayed excellent specificity and was successfully utilized to target GSH in real human serum samples. Importantly, this work not only highlights a powerful avenue for constructing an ultrasensitive ECL sensor for GSH but also provides some inspiration for the further design of high-performance co-reaction accelerators using the ECL technique.
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