An electrochemical aptasensor based on P-Ce-MOF@MWCNTs as signal amplification strategy for highly sensitive detection of zearalenone

In this research, a signal-off electrochemical aptasensor with high sensitivity was constructed for trace detection of zearalenone (ZEN). Specifically, Ce-based metal-organic framework and multi-walled carbon nanotubes nanocomposite was functionalized with polyethyleneimine (P-Ce-MOF@MWCNTs) and served as sensing platform for its high surface area and excellent electrochemical active. Subsequently, toluidine blue (TB) was electrodeposited as the signal probe, and platinum@gold nanoparticles (Pt@Au) were dropped for the attachment of aptamer (ZEA). In the presence of ZEN, the ZEA would specifically recognize and combine with the target, causing a decrease of electrochemical signal from TB. Under the optimal conditions, the aptasensor exhibited good linear relationship for ZEN in a concentration range from 5.0 × 10-5 to 50.0 ng/mL, while the limit of detection (LOD, S/N = 3) and limit of quantitation (LOQ, S/N = 10) were 1.0 × 10-5 ng/mL and 2.9 × 10-5 ng/mL, respectively. Ultimately, the aptasensor was successfully applied into ZEN detection in semen coicis real samples.