交叉展示
抗原
抗原呈递
细胞毒性T细胞
抗原提呈细胞
树突状细胞
CTL公司*
CD8型
免疫学
免疫系统
卵清蛋白
体内
体外
生物
细胞生物学
T细胞
癌症研究
生物化学
生物技术
作者
Pengju Ou,Lifen Wen,Hai Nie,Cliff Y Yang
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 239-249
标识
DOI:10.1007/978-1-0716-2938-3_17
摘要
Antigen cross-presentation by dendritic cells is an important pathway to prime CD8+ T cells in infections, cancer, and other immune-mediated pathologies. Particularly in cancer, cross-presentation of tumor-associated antigens is crucial for an effective antitumor CTL response. The mostly accepted cross-presentation assay is to use chicken ovalbumin (OVA) as a model antigen and then utilize OVA-specific TCR transgenic CD8+ T (OT-I) cells to measure the cross-presenting capacity. Here we describe in vivo and in vitro assays to measure the function of antigen cross-presentation using cell-associated OVA.
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