Combined effects of microplastics and antibiotic-resistant bacteria on Daphnia magna growth and expression of functional genes

大型水蚤 生物 微塑料 基因 微生物学 细菌 基因表达 氧化应激 遗传学 生物化学 化学 毒性 生态学 有机化学
作者
Xiaofeng Chen,Xiaocui Wang,Yijiang Huang,Ziying Zhu,T. J. Li,Zeming Cai,Minqian Li,Han Gong,Muting Yan
出处
期刊:Science of The Total Environment [Elsevier]
卷期号:905: 166880-166880
标识
DOI:10.1016/j.scitotenv.2023.166880
摘要

Microplastics could act as vectors for the transport of harmful bacteria, such as pathogens and antibiotic resistance bacteria (ARB), but their combined effects have not been reported yet. Here, ARB Shigella flexneri with sulfonamides resistance and micro-polystyrene (micro-PS) were used to investigate their possible combined effects on the growth and expression of functional genes in Daphnia magna. Results showed that micro-PS colonized with S. flexneri were ingested by D. magna and blocked in their intestine after 24 h exposure. Changes were observed in the life history and morphology of D. magna, as well as the expression of functional genes in all treatments, but with no difference in the survival rate. We also determined the expression of six functional genes involved in energy and metabolism (arginine kinase, AK) and oxidative stress response (thioredoxin reductase, TRxR, catalase, CAT, and glutathione S-transferases, GSTs), as well as in growth, development and reproduction (vitellogenin, Vtg1 and ecdysone receptor, EcR). AK and Vtg1 did not show significant differences, however, EcR was down-regulated and the other three genes (TRxR, CAT, GSTs) were up-regulated in the combined-treated group. Antibiotic resistance gene (ARGs) sul1 was detected when exposed to micro-PS colonized with S. flexneri., suggesting that D. magna could acquire resistance genes through microplastic biofilms. These results indicated that MPs could act as a carrier of ARB to transfer ARGs into D. magna, and affect the life history, morphology, and the expression of related functional genes of D. magna, to adapt to the stress caused by MPs and ARB.
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