清脆的
反式激活crRNA
计算生物学
生物
基因组编辑
致病菌
基因
DNA
CRISPR干扰
Cas9
遗传学
细菌
作者
Y. Wang,Tianmu Yang,Guifang Liu,Longfei Xie,Jianying Guo,Wenguang Xiong
标识
DOI:10.1016/j.cca.2023.117520
摘要
The combination of clustered regularly interspaced short palindromic repeats (CRISPR) and its associated Cas protein is an effective gene-editing instrument. Among them, the CRISPR-Cas12a system forms a DNA-cleavage-capable complex with crRNA and exerts its trans-cleavage activity by recognising the PAM site on the target pathogen's gene. After amplifying the pathogenic gene, display materials such as fluorescent probes are added to the detection system, along with the advantages of rapid detection and high sensitivity of the CRISPR system, so that pathogenic bacteria can be diagnosed with greater speed and precision. This article reviews the mechanism of CRISPR-Cas12a in rapid detection, as well as its progress in the rapid detection of pathogenic bacteria in conjunction with various molecular biology techniques, in order to provide a foundation for the future development of a more effective detection platform.
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