Determination of Ginsenoside-Rg1,Re and Rb1 in Different Panax Ginsengs

Objective:To establish a method for determination of Ginsenoside-Rg1、Re and Rb1 in Radix et Rhizoma Ginseng,Radix et Rhizoma Ginseng Rubra and Radix Panacis Quinquefolii by UPLC.Methods:The analysis was performed on Acquity UPLC BEH C18 column with column temperature set at 40 ℃.The mobile phase was composed of acetonitrile and water by gradient elution with a flow rate of 0.5 mL/min.The detection wavelength was at 203 nm.Results:Ginsenoside-Rg1、Re and Rb1 in Radix et Rhizoma Ginseng,Radix et Rhizoma Ginseng Rubra and Radix Panacis Quinquefolii were seperated completely in 14 minutes and were well analyzed by UPLC.Conclusion:The quantity of Ginsenoside-Rg1、Re and Rb1 in Radix et Rhizoma Ginseng,Radix et Rhizoma Ginseng Rubra and Radix Panacis Quinquefolii can be measured simultaneously by this method with the equipment of UPLC.Speeds of assays of Ginsenosides were greatly accelerated UPLC,and reagents used as mobile phases were also reduced greatly.The method using UPLC can provide a reference to analyze Ginsenosides.