The objective of the paper is to investigate the sulfur dioxide(SO2) to inhibit Botrytis cinera Pers to control the decay of grapes during storage to select suitable SO2 concentration inhibiting Botrytis cinera Pers to provide a bass for controlling decay of grapes during storage.The fuming treatment medium with different SO2 concentrations and Botrytis cinera Pers on the surface of grapes fruit were used to study inhibition effects on Botrytis cinera Pers and effects on grapes quality and to select suitable SO2 treatment concentration.Resuts showed that there was positive correlation between the inhibition effect of Botrytis cinera Pers in vitro and the inhibition and concentration of Botrytis cinera Pers.The significant effect of SO2 to control Botrytis cinerea Pers was beginning from 200 μL/L.Grapes inoculated Botrytis cinerea Pers bacteriostatic experiment showed,200 μL/L or 250 μl/L treatment effect was significantly better than the other concentrations.But 250 μl/L SO2 handling was harmful to grape quality.Suming up the inhibition of Botrytis cinerea Pers and the affection of grape fruit quality,SO2 concentration of 200 μL/L was suit for embalmed thompson seedless storage.