Preparation and Identification of Human Anti-Amyloid β Peptide Monoclonal Antibodies

Objective: To preparation anti-amyloid β peptide(Aβ) antibodies from human phage display antibody library.Methods: Aβ1-15 peptide was used for screening specific antibodies from a large fully synthetic human antibody library by solid panning.The antigen binding activity and specificity of antibody were determined by ELISA.Results: After 3 rounds of panning,one candidate(F11) was obtained.The epitope was located within Aβ1-15,and the specificity of F11 was shown to be excellent both in phage-antibody level and whole antibody level by ELISA.Conclusion: Human anti-Aβ1-15 specificity antibodies can be isolated from phage antibody library.