Cytotoxic activity of phenazine compounds Pseudomonas chlororaphis subsp. aurantiaca in relation to HeLa cell culture

In this work, the cytotoxic activity of phenazine compounds of different strains of Pseudomonas chlororaphis subsp. aurantiaca against the cervical adenocarcinoma cell line HeLa was investigated. The cytotoxic concentrations of phenazines against HeLa cells were 300 mcg/ml. Cytological preparations of HeLa cells were obtained after incubation with phenazines, showing the presence of apoptotic bodies. It was shown that the effect of phenazines on HeLa cells leads to a change in the expression of their ABC transporter genes (abcc1 and abcg2) and of tp53. The activity of tp53 increases almost 13-fold, while the expression of the abcc1 and abcg2 genes decreases. Activation of tp53 is probably one of the causes of apoptotic death of HeLa cells in the presence of phenazine compounds from Pseudomonas chlororaphis subsp. aurantiaca.