Targeted and Oxygen‐Enriched Nanoplatform for Enhanced Photodynamic Therapy: In Vitro 2D Cell and 3D Spheroid Model Evaluation

Abstract Hypoxic microenvironment and limited penetration of photosensitizers within solid tumors are two crucial factors that restrict photodynamic therapy (PDT) efficacy. Herein, a new fluorinated mixed micelle ( M60@PFC‐Ce6 ) is developed as a tumor‐penetrating and oxygen‐enriching nanoplatform, which consists of chlorin e6 (Ce6) and perfluorocarbons (PFCs) co‐loaded into fluorinated micelles to relieve hypoxia conditions as well as folate as targeting ligand that facilitates the selective biodistribution within tumor solids. The incorporation of fluorinated copolymers into mixed micelles exhibits not only a great increase in the oxygen‐loading capacity, but also improves the stability of liquid PFCs emulsion within micelles without leakage. M60@PFC‐Ce6 shows excellent oxygen delivery capability, good intracellular reactive oxygen species (ROS) generation, and superior phototoxicity in vitro for both 2D monolayer of cells and 3D multicellular spheroid model. These results indicate the enriched oxygen delivery and increased cellular uptake resulting from folate‐targeted ability to enhance ROS production and PDT efficacy. The penetration study of M60@PFC‐Ce6 into a 3D spheroid confirms that small micellar size and folate‐conjugation are beneficial for micelles to penetrate and accumulate within spheroids. Thus, a new nanoplatform with enriched oxygen‐carrying amounts, better drug penetration, and stable micellar properties that relieve tumor hypoxia and improve PDT efficacy is provided.