Medicago sativa and M. tunetana reveal contrasting physiological and metabolic responses to drought

Alfalfa production is frequently constrained by drought, indicating the importance of assessing species biodiversity in endemic close relatives to enhance forage production under future global change conditions. In the present study, plants of two ecotypes of M. tunetana, native to Tunisia, and four commercial cultivars of M. sativa were subjected to two water regimes (control vs drought [15% field capacity]). Physiological, isotopic and metabolic analyses were used to characterize leaf and nodule profiles of the plants. Biomass, gas exchange and the maximum carboxylation rate (Vcmax) indicated significant decreases in photosynthetic capacity under drought in M. sativa cultivars. However, M. tunetana ecotypes maintained photosynthetic performance and aboveground biomass under drought conditions. Furthermore, nitrogen isotope composition (δ15N) in nodules and leaves was significantly decreased, which reveals a reduction in the N2 fixing activity of nodules under drought conditions that was not translated into lower leaf N content but was probably due to lower N demand. Analyses of starch, soluble sugar, and amino acid content in leaves and nodules have clearly proven the ability of Medicago spp. cultivars to increase the accumulation of osmo-protectors under drought. This study demonstrated the genetic variability of the strategy adopted among the studied cultivars in response to drought. In this sense, M. tunetana, and in part the M. sativa cultivar adapted to Mediterranean conditions, seem capable of maintaining adequate biomass, photosynthesis and biological N2 fixation in comparison to the other M. sativa cultivars.