Fullerene-promoted organic-inorganic hybrid electrochemiluminescence biosensor for sensitive detection of concanavalin A

A fullerene-promoted organic-inorganic hybrid electrochemiluminescence (ECL) biosensor was proposed for sensitive detecting concanavalin A (ConA). Here, platinum-modified inorganic TiO2 (Pt-TiO2) promotes charge transfer to enhance ECL intensity of organic luminescent probe—ammonolysis product of PTCDA (PTC). Fullerene interacts with K2S2O8 co-reagent to produce more SO4•− free radicals, and its large specific-surface-area and electrophilic nature are beneficial to loading more luminescent PTC. The glucose oxidase (GOx) is chosen for specific recognition of ConA through a biospecific interaction. The constructed ECL biosensor shows a wide detection range of 1 × 10−4–1 × 103 ng mL−1 with a low detection limit (LOD) of 1.8 × 10−5 ng mL−1, as well as high stability, reproducibility, and detection capability of actual serum. This work provides a promising organic-inorganic hybrid biosensor platform for early biotherapy and clinical diagnosis.