The influence of elicitation on the tissue cultures of roseroot [Rhodiola rosea L.]

In this study the influence of the elicitation with yeast extract on the content of salidroside in the callus tissue of Rhodiola rosea was investigated. The colorimetric method for salidroside determination was used for quantitative analysis of this compound. Two series of experiments showed that the elicitor increased the salidroside content from 0.8% to 1.4% and from 0.8% to 1.5% respectively. The salidroside content in the elicited callus was twice as high as in five-year old roots of intact plants. Two types of pharmacological studies were used for the estimation of the adaptogenic activity of R. rosea extracts: determination of immunostimulating activity and determination of antistress effect.