Implementation of sourdough technology would increase nutritional value and attractiveness of gluten-free bread. However, fermentation process requires stimulation. The course of fermentation of gluten-free sourdoughs prepared from maize and soy flours and maize and potato starches, enriched in glucose and casein hydrolysate or soy sprouted seeds used as fermentation enhancers was evaluated. The clearest effect of the supplementation was observed in the case of sourdough prepared from starches only. The addition of glucose in the amounts as low as 0.5% has proved to be enough to enhance fermentation effectively. Supplementation of the sourdoughs with peptides was not very effective; however, when used together with glucose, a synergistic effect could be detected. The addition of sprouted seeds allowed to shorten the fermentation process with up to several hours. These studies have demonstrated possibility to simplify and shorten the process of gluten-free sourdough fermentation by modification of mixtures composition.