Strategies and methods for cloning plant genes

In the present,plant genes cloning was the front and focus in the field of botany research.After the development of about twenty years,some methods for cloning plant genes were established,which included functional cloning,PCR amplifing cloning,transposon or T DNA tagging,postional cloning,differential hybridization and subtractive hybridization,mRNA differential display and artificial synthesis DNA cloning.In this paper,these techniques theory,advantages and disadvantages of each,as well as their application prospects in cloning plant genes were introduced and dicussed.