Determination of Liquiritigenin and Glycyrrhizic Acid in Xuanmaiganjie Granule by HPLC

OBJECTIVE To establish an HPLC method for the determination of liquiritigenin and glycyrrhizic acid in Xuanmaiganjie granule.METHODS A LiChrospher C18column(250 mm×4.6 mm,5 μm) was used,the mobile phase was acetonitrile-water(1.2% methanoic acid) for gradient elution.The detection wavelength was set at 254 nm. RESULTS The linear range was 0.005-0.5 mg·mL-1.The regression equation of liquiritigenin was Y=5 735.789 7X-2.702 7(r=0.999 9),and the average recovery was 99.7%(RSD=1.72%,n=9).The regression equation of glycyrrhizic acid was Y=6 968.182 6X-3.448 4(r=0.999 8),and the average recovery was 100.8%(RSD=1.31%,n=9).CONCLUSION The method is convenient and accurate.It can be used to determine the contents of liquiritigenin and glycyrrhizic acid in Xuanmaiganjie granule.