Three kinds of liquid fluorescent removers, the UK produced, German L1 and German L2 were investigated respectively. To discuss their fluorescent elimination effects, the liquid fluorescent remover dosage, mixed temperature and mixed time were studied respectively. The experimental results showed that the remover agent type, remover agent dosage, mixing temperature and mixing time have great influences on the fluorescence eliminating effects. Among the three kinds of liquid fluorescent removers, the UK produced performs best. With mixed temperature at 30℃, mixed time for 10 min and consumption dosage only 0.5% of oven dry pulp, the fluorescent whiteness F was reduced by 6.2%, while R457 whiteness lost only 4.7%ISO. Characterizing the fluorescence effects through infrared spectrum and scanning electron microscopy, and the results show that the UK produced liquid fluorescent removing agent can effectively eliminate fluorescent substances from the slurry.