抗菌剂
银纳米粒子
多重耐药
化学
醋酸
琼脂扩散试验
核化学
大肠杆菌
微生物学
纳米颗粒
生物
生物化学
抗生素
纳米技术
有机化学
材料科学
基因
作者
Rohini Pungle,Shivraj Hariram Nile,Arun S. Kharat
摘要
Plant extracts and their bioactive compounds are considered as the promising options for green synthesis of nanoparticles instead expensive and hazardous materials. Here, Solanum xanthocarpum fruit was used for synthesis of silver nanoparticles (AgNP). The synthesized AgNPs were characterized by using chromatographic and spectroscopic analytical methods. AgNPs were confirmed by UV-visible absorbance at 420-470 nm. TEM analysis showed AgNP with 22.45 nm average size. X-ray diffraction studies revealed the crystalline and face central cubic nature of AgNPs. FTIR analysis revealed functional group present over AgNPs. The aminodiphenyl acetic acid, clomipramine, and fonisopril from fruit extracts were found to be major capping agents on AgNPs as a result of analysis by HRLC-MS. All clinical isolates showed resistance for ampicilline, amoxyclav, niladixic acid, and sulphafurazole, suggesting multidrug resistance. The results showed that all isolates were sensitive to AgNPs synthesized fruit extracts. On the contrary, all isolates were resistant to whole S. xanthocarpum fruit extracts alone. The antimicrobial activity of AgNP was explored against multidrug-resistant (MDR) Gram-negative clinical isolates including Escherichia coli, Shigella spp., Aeronomonas spp. and Pseudomonas spp. MIC values ranged between 1.25 mg/ml and 2.5 mg/ml at 8 McFarland's standards. Minimum bactericidal concentration was found to be in between 2.5 mg/ml to 5 mg/ml. Nanoparticles synthesized from fruit extract of S. xanthocarpum containing aminodiphenyl acetic acid, clomipramine, and fonisopril metabolites exhibit promising antimicrobial activity against MDR Gram-negative clinical isolates.
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