针灸科
导水管周围灰质
创伤性脑损伤
多巴胺能
神经科学
多巴胺
医学
彗差(光学)
TRPV1型
心理学
麻醉
中脑
药理学
内科学
受体
病理
中枢神经系统
精神科
瞬时受体电位通道
物理
替代医学
光学
作者
Huiling Tang,Siru Qin,Wei Li,Xuyi Chen,Luis Ulloa,Qiumei Zhu,Baohu Liu,Yinan Gong,Yadan Zhao,Songtao Wang,Shanshan Li,Yongming Guo,Zhifang Xu,Yi Guo
标识
DOI:10.3389/fncel.2020.598198
摘要
The induction of a coma by traumatic brain injury (TBI) is a crucial factor for poor clinical prognoses. We report that acupuncture at the hand 12 Jing -Well points (HTWP) improved consciousness and neurologic function in TBI rats. Gene chip analyses showed that HTWP acupuncture mostly activated genes modulating neuronal projections ( P2rx7, P2rx3, Trpv1, Tacr1 , and Cacna1d ), protein secretion ( Exoc1, Exoc3l1, Fgb , and Fgr ), and dopamine (DA) receptor D3 ( Drd3 ) in the ventral periaqueductal gray (vPAG), among which the expression rate of P2rx7 was the most obviously increased. Acupuncture also increased the expression and excitability of DA and P2RX7 neurons, and the DA neurons expressed P2RX7, P2RX3, and TRPV1 in the vPAG. Intracerebroventricular administration of P2RX7, P2RX3, or TRPV1 antagonists blocked acupuncture-induced consciousness, and the subsequent injection of a P2RX7 antagonist into the vPAG nucleus also inhibited this effect. Our findings provide evidence that acupuncture alleviates TBI-induced comas via DA neurons expressing P2RX7 in the vPAG, so as to reveal the cellular and molecular mechanisms of the improvement of TBI clinical outcomes by HTWP acupuncture.
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