叶绿体
警卫室
拟南芥
胞浆
光合作用
类囊体
化学渗透
ATP合酶
细胞生物学
线粒体
ATP酶
生物化学
叶绿素
三磷酸腺苷
化学
生物物理学
生物
突变体
基因
酶
作者
Shey-Li Lim,Sabrina Flütsch,Jinhong Liu,Luca Distefano,Diana Santelia,Boon Leong Lim
标识
DOI:10.1038/s41467-022-28263-2
摘要
Abstract Stomatal opening requires the provision of energy in the form of ATP for proton pumping across the guard cell (GC) plasma membrane and for associated metabolic rearrangements. The source of ATP for GCs is a matter of ongoing debate that is mainly fuelled by controversies around the ability of GC chloroplasts (GCCs) to perform photosynthesis. By imaging compartment-specific fluorescent ATP and NADPH sensor proteins in Arabidopsis, we show that GC photosynthesis is limited and mitochondria are the main source of ATP. Unlike mature mesophyll cell (MC) chloroplasts, which are impermeable to cytosolic ATP, GCCs import cytosolic ATP through NUCLEOTIDE TRANSPORTER (NTT) proteins. GCs from ntt mutants exhibit impaired abilities for starch biosynthesis and stomatal opening. Our work shows that GCs obtain ATP and carbohydrates via different routes from MCs, likely to compensate for the lower chlorophyll contents and limited photosynthesis of GCCs.
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