芹菜素
体内
传统医学
黄芩
木犀草素
肝细胞癌
体外
细胞生长
乙型肝炎病毒
细胞培养
诃子
药理学
化学
医学
生物
病毒
生物化学
病毒学
类黄酮
中医药
癌症研究
抗氧化剂
替代医学
生物技术
病理
遗传学
作者
Peiwei Yang,Tingting Chen,Wenxia Zhao,Guangwei Liu,Xiaohai Feng,Shou‐Mei Wang,Yun‐Cui Pan,Qian Wang,Shu‐Hui Zhang
标识
DOI:10.1016/j.jep.2021.114110
摘要
Scutellaria barbata D.Don (SB) and Oldenlandia diffusa (Willd.) Roxb are commonly known as Ban Zhi Lian and Bai Hua She Cao in Chinese herbal medicines, respectively. As a pair of herbs, they have traditionally been used as ethnomedicines for clearing away heat and toxins, removing blood stasis, and promoting blood circulation, diuresis, and detumescence. The aim of the present study was to determine the active ingredients in SB and OD extracts and to investigate whether these extracts can inhibit the growth of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) cell lines (HepG2.2.15 and Hep3B) in vitro and in vivo, as well as to explore their mechanisms of action. We determined the levels of total flavonoids, luteolin, and apigenin in SB and OD extracts via ultraviolet–visible spectrophotometry and high-performance liquid chromatography. The effects of SB and OD extracts on HBV-associated HCC cell growth were assessed by HepG2.2.15 and Hep3B cells phenotype and RNA sequencing of Hep3B cells in vitro, and xenograft models in vivo. The extracts of SB and OD contained total flavonoids. There were active ingredients of luteolin and apigenin in SB, but not in OD. The extracts of SB and OD significantly inhibited HCC growth, migration, invasion, and HBV activity in vitro and in vivo, as well as altered circRNA expression in Hep3B cells. Moreover, we constructed a circRNA-miRNA-mRNA co-expression network. The extracts of SB and OD may inhibit HCC cell growth and HBV activity in vitro and in vivo through altering circRNA-miRNA-gene expression and that the efficacies of these extracts may be related to the presence of luteolin and apigenin.
科研通智能强力驱动
Strongly Powered by AbleSci AI