Evolutions in Science Triggered by Green Fluorescent Protein (GFP)

Painting by proteins: Based on green fluorescent protein (GFP), other fluorescent proteins were developed, which differ from normal GFP by their fluorescence characteristics, stability, maturation, or pH sensitivity (the 3D structure of enhanced GFP (EGFP) is shown). These proteins opened up a vast field of potential applications, including live-cell fluorescence microscopy, visualization of protein interactions, and investigation of a great variety of cellular parameters.