肝细胞癌
细胞凋亡
流式细胞术
干扰素
细胞周期
癌症研究
细胞培养
细胞
生物
细胞内
细胞生长
免疫学
细胞生物学
生物化学
遗传学
作者
Masayuki Murata,Shigeki Nabeshima,K. Kikuchi,Kouzaburo Yamaji,Norihiro Furusyo,Jun Hayashi
出处
期刊:Cytokine
[Elsevier]
日期:2006-02-07
卷期号:33 (3): 121-128
被引量:44
标识
DOI:10.1016/j.cyto.2005.08.011
摘要
The antiviral, antiproliferative and immunomodulatory effects of type I interferons (IFNs) are well documented, however, few studies have been published concerning differences in the antitumor effects of IFN-α and β. In the present study, differences in antitumor effect, including the antiproliferative effect, cell cycle change, apoptosis, and the IFN-stimulated gene (ISG) were examined by flow cytometry between IFN-α and β on three human hepatocellular carcinoma (HCC) cell lines (HepG2, Huh7 and JHH4). The antiproliferative effect of both IFNs on the HCC cell lines was time- and dose-dependent, and IFN-β was significantly stronger than IFN-α. The cell cycle effect by both IFNs was an S-phase accumulation, with IFN-β having a tendency to increase the S-phase ratio more strongly than IFN-α, especially in Huh7. Apoptosis marker expression, Fas antigen and intracellular active caspase-3, was increased after the addition of IFNs, especially of IFN-β. The expression of human leukocyte antigen-class I molecules, ISG-encoded protein, was increased after the addition of IFNs, especially of IFN-β. These data suggest that IFN-β has a greater antitumor effect than IFN-α on HCC of a very early stage in patients with chronic hepatitis C.
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