底漆(化妆品)
生物
DNA
线粒体DNA
物种鉴定
动物种类
聚合酶链反应
禽肉
16S核糖体RNA
鉴定(生物学)
食品科学
计算生物学
遗传学
动物
植物
化学
基因
有机化学
作者
Stefanie Dobrovolny,Marion Blaschitz,Thomas Weinmaier,Jan Pechatschek,Margit Cichna‐Markl,Alexander Indra,Peter Hufnagl,Rupert Hochegger
标识
DOI:10.1016/j.foodchem.2018.08.032
摘要
Meat products are prone to adulteration by the replacement of meat from more expensive animal species with meat from cheaper sources. We present a DNA metabarcoding method allowing the identification and differentiation of 15 mammalian and six poultry species in foodstuffs. The method, developed on the MiSeq® platform, targets a mitochondrial 16S rDNA region recently found to be suitable for the differentiation of 300 mammalian species. We designed a novel primer pair for poultry and applied it in combination with the primer pair for mammalian species in a duplex assay. The applicability of the method was investigated by analysing DNA extracts from muscle, DNA extract mixtures and extracts from model sausages. Our results indicated that the species of interest can be identified, differentiated and detected down to a proportion of 0.1%. Since 96 samples can be sequenced in one run, the method has high potential for application in routine analysis.
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