Impact of Heavy Chain C-Terminal Codons on Recombinant Monoclonal Antibody Expression Levels.

was found to broadly influence the expression of other industrially relevant IgG mAb isotypes, including IgG2 and IgG4. This study establishes a connection between the HC C-terminal nucleotide sequence and mAb expression titer, offering insights for optimizing the expression of Fc-containing molecules.