STUDY ON VARIATION OF VIRULENCE GENES OF FOODBORNE LISTERIA MONOCYTOGENES IN HENAN PROVINCE

[Objective]Variation patterns and distribution of virulence genes of foodborne Listeria Monocytogenes in Henan Province were studied to ensure food safety.[Methods]Polymerase Chain Reaction was used to identify 8 virulence including prfA,plcA,hly,mpl,actA,plcB,inlA and inlB,and one virulence related gene(iap)from 14 and 42 Listeria Monocytogenes which were isolated in 2007 and 2009,as well as 10 Listeria Innocua isolates which were recovered in 2009.[Results]Nine virulence genes were all identified in 14 Listeria Monocytogenes isolated in 2007. Thirty-seven of 42 Listeria Monocytogenes were all positive to 9 virulence genes compared to 5 isolates were deficient to some of above mentioned genes including lap,prfA,inlB,hlyA and mpl. Gene deficiency had no relation to food type. One of 10 Listeria Innocua isolates was detected to harbor plcB and iap genes,and no gene were detected in other 9 strains.[Conclusion]Nine virulence genes were almost can be detected in foodborne Listeria Monocytogenes in Henan province,Virulence genes deficiency in Listeria Monocytogenes in natural condition can be occurred.