Effects of Different Feeders on Cultured Chicken Embryonic Stem Cells

Objective:To research the optimal culture condition of chicken embryonic stem cells,compare the effect of different feeders on cultured chicken embryonic stem cells in vitro.Methods:The second generation chicken embryonic fibroblast(CEF) and duck embryonic fibroblast(DEF) were used to make feeders after treated with mitomycin C.The state of chicken embryonic stem cells were observed and the number of cell clones was counted on the culture system with those two feeder layers or feeder layer free.Results:The results showed that chicken embryonic stem cells growth well both on the CEF and DEF feeder,and the number of cell clones was no distinct difference between CEF and DEF(P0.05).Conclusion:The results indicated that both of CEF and DEF can be used as feeders to culture chicken embryonic stem cells.