The Fluorescence Characteristic of AO-RB-SDS-GMFX and the Determination of Gemifloxacin

In Britton Robinson (B-R) buffer solution with pH = 7.00 and sodium dodecyl sulfate (SDS) medium, an effective energy transfer between acridine orange (AO) and Rhodamine B (RB) can occur, which can enhance the fluorescence of RB. The addition of Gemifloxacin (GMFX) can quench the fluorescence of RB. So a new method for the indirect determination of Gemifloxacin was established by AO-RB fluorescence resonance energy transfer. This method was applied to the determination of Gemifloxacin tablets. The results were consistent with those of high performance liquid chromatography (HPLC) . Experiments show that this method is simple, rapid, accurate and sensitive.