The expressions of CCL28 in the gastrointestinal tract of 15-day-old and 30-day-old piglets were determined by using RT-PCR method.The results showed that CCL28 mRNA was expressed in duodenum,ileum,cecum,colon,rectum of 15-day-old piglet and in duodenum,jejunum,ileum,cecum,colon,rectum and stomach tissues of 30-day-old piglet;no expression in duodenum,jejunum and stomach tissues,a little in ileum,colon tissues of 15-day-old piglet and in duodenum,jejunum,cecum,stomach tissues of 30-day-old piglet.The time-space expression of CCL28 showed that it was related to the gastrointestinal mucosal immune system development in the piglets and roles in different tissues.