The effect of bio-enzymatic treatment on the refining of bamboo fiber was studied in this paper.An orthogonal experiment was designed to optimize the process parameters of xylanase treatment.The weight loss and the residual gum content were measured.The results showed that the optimum parameters for bamboo fiber degumming were:temperature was 65℃,dosage 0.10g/ml,pH value 8.0 and treated time 180 minutes.The weight loss and residual gum content of the degummed fiber were 8% and 32.7% respectively.