Effects of iNOS inhibitor 1400 W on the cervical cancer cell line HeLa

Objective To explore the effects of iNOS inhibitor 1400 W alone or combined with cisplatin to cervical cancer cell line HeLa. Methods Cervical cancer cell line HeLa was resuscitated and incubated,MTT was used to investigate 1400 W and cisplatin’s effects on HeLa cell growth; flow cytometry was used to detect the HeLa cell apoptosis rate and RT-PCR was used to detecte the E6, p53 mRNA expression of HeLa cell. Results Both cisplatin and 1400 W showed a dose-dependent inhibitory effect on HeLa cell proliferation(P0.05). 1400 W could not increase HeLa cell’s apoptosis, while 1400 W combined cisplatin could both induce apoptosis and decrease E6, p53 mRNA expression of HeLa cell(P0.05). Conclusions 1400 W combined with ciplatin can increase the HeLa cell apoptosis rate than the group cisplatin used alone.Combined with iNOS inhibitor, it can enhance the cisplatin effect on tumor cell. This is possible a promising adjuvant therapy for cervical caner.