Study on the Determination Method for Aggressiveness of Sclerotinia sclerotiorum

[Objective] Determination methods of the aggressiveness of Sclerotinia sclerotiorum were explored.[Method] Effects of 4 methods(9 treatments) like in vivo leaf inoculation method with mycelial wafer,in vitro leaf inoculation method with mycelial wafer,in vivo stem inoculation method with mycelial wafer and the in vivo stem inoculation method with mycelial toothpick on the aggressiveness determination of Sclerotinia sclerotiorum were studied.[Result] All the 4 methods could distinguish the difference in aggressiveness of S.sclerotiorum,among which the in vivo leaf inoculation method with mycelial wafer without puncture was the most simple,rapid and accurate inoculation method for the aggressiveness test of S.sclerotiorum.[Conclusion] The research provides theoretical basis for the control of sclerotinia stem rot and the breeding of resistant varieties.