Determination of Fat-Soluble Vitamins by High-Performance Liquid Chromatography Tandem Mass Spectrometry in Feed, Feed Additives

Abstract This article describes the development of a technique for the determination of fat-soluble vitamins using high-performance liquid chromatography with mass spectrometric detection in feed, feed additives. Measurement ranges of mass concentrations of vitamins: A—from 0.35–14.0 mg/kg, D3—from 0.0065–1.0 mg/kg, and E—from 0.63–50.0 mg/kg. The technique is based on alkaline hydrolysis of the sample and extraction of fat-soluble vitamins with hexane. The analysis of the obtained extracts is carried out by high-performance liquid chromatography with mass spectrometric detection (HPLC MS/MS).