血流
生物医学工程
微流控
血流动力学
灌注
充氧
材料科学
微循环
体内
微通道
纳米技术
生物
医学
心脏病学
内科学
生物技术
作者
Rui Dong,Sijia Liu,Yuewu Li,Yongzhe Fan,Keqiang Gao,Chunxiao Chen,Zhiyu Qian,Weitao Li,Yamin Yang
标识
DOI:10.1016/j.mvr.2023.104640
摘要
The complexity of microvascular circulation has led to the development of advanced imaging techniques and biomimetic models. This study developed a multifaceted microfluidic-based microdevice as an in vitro model of microvasculature to replicate important geometric and functional features of in vivo perfusion in mice. The microfluidic device consisted of a microchannel for blood perfusion, mirroring the natural hierarchical branching vascular structures found in mice. Additionally, the device incorporated a steady gradient of oxygen (O2) which diffused through the polydimethylsiloxane (PDMS) layer, allowing for dynamic blood oxygenation. The assembled multi-layered microdevice was accompanied by a dual-modal imaging system that combined laser speckle contrast imaging (LSCI) and intrinsic signal optical imaging (ISOI) to visualize full-field blood flow distributions and blood O2 profiles. By closely reproducing in vivo blood perfusion and oxygenation conditions, this microvasculature model, in conjunction with numerical simulation results, can provide quantitative information on physiologically relevant hemodynamics and key O2 transport parameters that are not directly measurable in traditional animal studies.
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