Four New Flavonoid C‐Glycosides Isolated from Achyranthes aspera and Their Nitric Oxide Production Inhibitory Activities

Abstract A chemical study of the methanol extract of the aerial parts of Achyranthes aspera led to the isolation of four new flavonoid C ‐glycosides ( 1 – 4 ) along with eight known analogs ( 5 – 12 ). Their structures were elucidated by a combination of spectroscopic data analysis, HR‐ESI‐MS, 1D and 2D NMR spectra. All the isolates were evaluated their NO production inhibitory activity in LPS‐activated RAW264.7 cells. Compounds 2 , 4 , and 8 – 11 showed significant inhibition with IC 50 values ranging from 25.06 to 45.25 μM, compared to that of the positive control compound, L‐NMMA, IC 50 value of 32.24 μM, whereas the remaining compounds were weak inhibitory activity with IC 50 values over 100 μM. This is the first report of 7 from Amaranthaceae family, and 11 from the genus Achyranthes .