脂肪细胞
细胞生物学
脂滴
生物
脂肪组织
生物化学
作者
Jiwon Kim,Kun-Young Park,Sung-Woo Choi,Ung Hyun Ko,Dae‐Sik Lim,Jae Myoung Suh,Jennifer Hyunjong Shin
出处
期刊:Lab on a Chip
[The Royal Society of Chemistry]
日期:2022-01-01
卷期号:22 (20): 3920-3932
被引量:2
摘要
Adipocyte dedifferentiation has recently gained attention as a process underpinning adipocyte plasticity; however, a lack of suitable experimental platforms has hampered studies into the underlying mechanisms. Here, we developed a microscope-mountable ceiling culture chip that provides a stable yet tunable culture environment for long-term live-imaging of dedifferentiating adipocytes. A detailed spatiotemporal analysis of mature adipocyte dedifferentiation utilizing the culture platform and Cre-recombinase tracers revealed the involvement of dynamic actin remodeling for lipid droplet (LD) secretion during adipocyte dedifferentiation. Additionally, Hippo, Hedgehog, and PPARγ signaling pathways were identified as potent regulators of adipocyte dedifferentiation. Contrary to the belief that adult adipocytes are relatively static, we show that adipocytes are very dynamic, relying on actin-driven mechanical forces to execute LD extrusion and intercellular LD transfer processes.
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