Fast and easy immunomagnetic positive selection of PE- or biotin-conjugated antibody-labelled cells with Releasable Rapidspheres results in highly purified, functional and particle-free cells

Abstract Immunomagnetic cell isolation is a cost-effective and gentle method of obtaining purified cell populations. However, isolating novel or rare cell populations often relies on relatively time consuming and expensive cell sorting techniques due to the lack of off-the-shelf immunomagnetic cell isolation kits. To bridge this gap, we have developed the EasySep™ Release isolation kits for PE- or biotin-conjugated antibodies. EasySep™ Release uses magnetic particles with low non-specific binding characteristics that can be rapidly removed from isolated cells after positive selection. The system is highly customizable, targeting cells through the use of any biotin or PE-conjugated primary antibody or ligand. Starting with a variety of sample materials, including single cell suspensions from mouse spleen, lymph nodes, lungs, rat spleen, or human PBMCs, the EasySep™ Release kits have demonstrated purities consistently above 90%. The EasySep™ Release technology can also be used to isolate subsets of cells when used in combination with our other cell separation products. For example, functional and particle-free mouse CD25+ or CD304+ CD4+ regulatory T cells were isolated by using our EasySep™ PE Positive Releasable Rapidspheres™ kit and our EasySep™ Mouse CD4+ T Cell Isolation kit. We have used EasySep™ Release with EasySep™ Dextran Rapidspheres™ to isolate CD4/CD8 double positive mouse thymocytes (double positive selection), and to isolate mouse lung epithelial cells by EpCAM positive selection followed by the removal of contaminating leukocytes via CD45 depletion. These kits offer a customizable, and cost-effective magnetic isolation approach for the isolation of virtually any cell population.