微塑料
肠道菌群
粘液
微生物学
微生物群
生物
摄入
粪便
肠杆菌科
丁酸盐
食品科学
免疫学
生物化学
生态学
大肠杆菌
生物信息学
发酵
基因
作者
E Fournier,Jérémy Ratel,Sylvain Denis,Mathilde Lévêque,Philippe Ruiz,Carine Mazal,Frederic Amiard,Mathieu Edely,Valérie Bézirard,Eric Gaultier,Bruno Lamas,Eric Houdeau,Erwan Engel,Fabienne Lagarde,Lucie Etienne‐Mesmin,Muriel Mercier‐Bonin,Stéphanie Blanquet‐Diot
出处
期刊:Social Science Research Network
[Social Science Electronic Publishing]
日期:2022-01-01
被引量:13
摘要
Infants are characterized by an immaturity of the gut ecosystem and a high exposure to microplastics (MPs) through diet, dust and suckling. However, the bidirectional interactions between MPs and the immature infant intestinal microbiota remain unknown. Our study aims to investigate the impact of chronic exposure to polyethylene (PE) MPs on the gut microbiota and intestinal barrier of infants, using the new Toddler mucosal Artificial Colon coupled with a co-culture of epithelial and mucus-secreting cells. Gut microbiota composition was determined by 16S metabarcoding and microbial activities were evaluated by gas, short chain fatty acid and volatolomics analyses. Gut barrier integrity was assessed via evaluation of intestinal permeability, inflammation and mucus synthesis. Exposure to PE MPs induced gut microbial shifts increasing α-diversity and abundance of potentially harmful pathobionts, such as Dethiosulfovibrionaceae and Enterobacteriaceae. Those changes were associated to butyrate production decrease and major changes in volatile organic compounds profiles. In contrast, no significant impact of PE MPs on the gut barrier, as mediated by microbial metabolites, was reported. For the first time, this study indicates that ingestion of PE MPs can induce perturbations in the gut microbiome of infants. Next step would be to further investigate the potential vector effect of MPs.
科研通智能强力驱动
Strongly Powered by AbleSci AI